"Get information from raw data"

Lightsheet Z.1

Registration & fusion of octopus-images

With a ZEISS Lightsheet Z.1 of Carl Zeiss microscopy GmbH images of an octopus has been acquired by a team of scientists at the Okinawa Institute of Science and Technology from different angles and with different lighting directions. These eight image data (about 30 GB) were registered and fused from da-cons. Furthermore, this sample was 3D printed and given the name "Oscar".


Octopus bimaculoides (1 month old) , Alexa 546 phalloidin (actin/muscle), ToPro3-Alexa 642(DNA) , Acquired one a Lightsheet Z.1 Light Sheet Fluorescence Microscope by ZEISS ; Views: 4 , Dual Side Illumination , Multiview registration and fusion ; Data by Eric Edsinger & Daniel S. Rokhsar, Okinawa Institute of Science and Technology.

 

Registration & fusion of zebrafish-images

With a ZEISS Lightsheet Z.1 of Carl Zeiss microscopy GmbH images of a zebrafish has been acquired by a team of scientists at the MPI-CBG from four different angles and with different lighting directions (about 80 GB per timestep). To facilitate the registration, artificial beads are added nowadays. This will impede the process of sample preparation, image acquisition and image analysis. However, da-cons is succeeded to realize the registration WITHOUT these artificial pearls. Consequently, experiments and recordings by ZEISS Lightsheet Z.1 would be conceivable without beads and with the methods of as-cons.

The following figure shows 2D slices from two different & registered data sets and the subsequently following data fusion compared.

Zebrafish Embryo, 3 to 4 days old, HIS-RFP labels nuclei, ath5-gfp labels a transcription factor that has a role in regulating neurogenesis in the retina, Acquired on a Lightsheet Z.1 by Carl Zeiss Sample courtesy of Karen Norden / Jaroslav Icha, MPI-CBG, Dresden, Germany